Conjugation

Part:BBa_K1682014:Design

Designed by: Zilu Peter Tang   Group: iGEM15_HKUST-Rice   (2015-09-08)

Conjugation(RP4) plasmid with partial GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 638
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1015
    Illegal NgoMIV site found at 1141
    Illegal NgoMIV site found at 1151
    Illegal AgeI site found at 450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 672
    Illegal SapI site found at 1043


Design Notes

The designed plasmid allow conjugation between the E. coli with most other bacteria.


Source

RP4 is orignially from pRZ101, and it includes both BBa_J01001 and BBa_K125320. GFP is from part of the biobrick BBa_E0040. The RBS is from BB0030 and the terminator is from BB0014

References